Identification of Suitable Meloidogyne spp. Housekeeping Genes
نویسندگان
چکیده
منابع مشابه
Identification of genes from flat oyster Ostrea edulis as suitable housekeeping genes for quantitative real time PCR.
Bonamia ostreae is an intrahaemocytic protozoan affecting Ostrea edulis. The parasite multiplies within haemocytes without being degraded and involves changes in cellular activities. Studies aiming at better understanding host response to a pathogen at the transcriptome levels are frequently based on the use of real time PCR assays, which require some reference genes. However, very few sequence...
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Identification of species of Meloidogyne Goeldi, 1887 is fi-equently accomplished by using perineal patterns and morphometrics, but intraspecific populations are not easily distinguished. Chromosome numbers have been determined for 10 (6) of the 36 species of Meloidogyne which are currently recognized (1, 5). Four of these species have a range of chromosome numbers. This chromosome range permit...
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Enolases are highly conserved proteins that catalyze the interconversion of 2-phosphoglyceric acid and phosphoenolpyruvate [1]. This is an essential step during glycolysis as well as gluconeogenesis. Interestingly, evolution has incorporated multiple novel non-glycolytic functions in several glycolytic enzymes (Reviewed in [2]). Such non-glycolytic or ‘moonlighting functions’ of enolase range f...
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Injury to the peripheral nerve induces dramatic changes in terms of cellular composition that are reflected on RNA quality and quantity, making messenger RNA expression analysis very complex. Several commonly used housekeeping genes are regulated following peripheral nerve injury and are thus not suitable for quantitative real-time PCR normalization; moreover, the presence of pseudogenes in som...
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The searching of human housekeeping (HK) genes has been a long quest since the emergence of transcriptomics, and is instrumental for us to understand the structure of genome and the fundamentals of biological processes. The resolved genes are frequently used in evolution studies and as normalization standards in quantitative gene-expression analysis. Within the past 20 years, more than a dozen ...
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ژورنال
عنوان ژورنال: journal of nematology
سال: 2019
ISSN: 0022-300X
DOI: 10.21307/jofnem-2019-055